Process for detecting evanescently excited luminescence

Details Process for detecting evanescently excited luminescence Process for detecting evanescently excited luminescence

G - Physics

01

N

G01N 21/77 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2190362

The invention relates to a process for detecting luminescence with a planar dieletric optical sensor platform consisting of a transparent substrate (a) to which a thin transparent waveguiding layer (b) is applied, which sensor platform is provided with a grating for the input- coupling of the excitation light and the refractive index of said substrate (a) is lower than the refractive index of the waveguiding layer (b), by bringing a liquid sample into contact with the layer (b), and measuring the luminescence produced by substances having luminescence properties in the sample, or by substances having luminescence properties immobilised on the layer (b), optoelectronically. The invention also relates to the use of the process in quantitative affinity sensing and to the use thereof for the quantitative determination of luminescent constituents in optically turbid solutions.

L'invention se rapporte à un procédé de détection de luminescence au moyen d'une plaque de détection optique diélectrique plane composée d'un substrat transparent (a) auquel est appliquée une couche de guidage d'ondes transparente et mince (b), cette plaque étant pourvue d'une grille permettant le couplage d'entrée de la lumière d'excitation, et l'indice de réfraction dudit substrat (a) étant inférieur à celui de la couche de guidage d'ondes (b). Ce procédé consiste à mettre un échantillon liquide en contact avec la couche (b), et à mesurer de manière optoélectronique la luminescence produite par des substances présentant des propriétés luminescentes dans l'échantillon, ou par des substances présentant des propriétés luminescentes immobilisées sur la couche (b). L'invention concerne par ailleurs l'utilisation de ce procédé pour la détection quantitative par affinité et pour la détermination quantitative des constituants luminescents dans des solutions optiquement troubles.

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