Process for detecting evanescently excited luminescence

G - Physics – 01 – N

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G01N 21/77 (2006.01) G01N 21/03 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2190643

The invention relates to a process for determining luminescence with a planar dielectric optical sensor platform which consists of a transparent substrate (a) to which a thin transparent waveguiding layer (b) is applied, which sensor platform is provided with a coupling grating for the input-coupling of the excitation light and the refractive index of said substrate (a) is lower than the refractive index of the waveguiding layer (b), by bringing a liquid sample as superstrate into contact with the layer (b), and measuring the luminescence produced by substances having luminescence properties in the sample, or by substances having luminescence properties immobilised on the layer (b) optoelectronically. The invention also relates to the use of the process in quantitative affinity sensing and to the use thereof for the quantitative determination of luminescent constituents in optically turbid solutions, and to a sensor platform for carrying out said process.

Un procédé permet de détecter une luminescence avec une plaque de détection optique diélectrique plane composée d'un substrat transparent (a) auquel est appliquée une mince couche transparente (b) servant de guide d'ondes. Cette plaque de détection est dotée d'un sélecteur de mode de couplage destiné au couplage en entrée de la lumière d'excitation, l'indice de réfraction du substrat (a) étant inférieur à celui de la couche en guide d'ondes (b). Ce procédé consiste à amener un échantillon liquide en forme de strate supérieure en contact avec la couche (b) et à mesurer de façon opto-électronique la luminescence produite par des substances à propriétés luminescentes présentes dans l'échantillon ou immobilisées sur la couche (b). L'invention concerne aussi l'utilisation de ce procédé pour la détection quantitative à affinité et pour la détermination quantitative de constituants luminescents dans des solutions optiquement troubles, ainsi qu'une plaque de détection permettant d'appliquer ce procédé.

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