Process for detecting p53-specific antibodies

Details Process for detecting p53-specific antibodies Process for detecting p53-specific antibodies

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 7/08 (2006.01) C07K 14/42 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12N 15/11 (2006.01) G01N 33/566 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2124595

Method of Detecting p53-Specific Antibodies The invention relates to a method of detecting p53- specific antibodies in body fluids wherein support-bound p53 and/or support-bound fragments thereof comprising binding regions for p53-specific antibodies are incubated with body fluids and the specific antibodies (a) bound to p53 and/or said fragments are allowed to react - with labelled antibodies (b) directed against antibodies (a), or - with unlabelled antibodies (b) while the latter are allowed to react with labelled antibodies (c) directed against antibodies (b), with the labelling being non- reactive in either case. The invention further relates to a kit useful therefor. Also, the invention relates to p53 fragments and DNA sequences coding for them, said fragments comprising binding regions for p53-specific antibodies, as well as to methods of preparing them.

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