Process for detecting specific nucleotide variations and...

Details Process for detecting specific nucleotide variations and... Process for detecting specific nucleotide variations and...

C - Chemistry, Metallurgy

12

Q

150/3.3, 150/8.5

C12Q 1/68 (2006.01) C07K 14/74 (2006.01)

Patent

CA 1284931

Abstract of the Disclosure Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.

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