G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 33/74 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2143656
A process and appropriate device are disclosed for determining the androstenone content of adipose tissues in clearly shor- ter times. The disclosed process has the following steps: (a) liquefying an adipose tissue sample by heating up to a predetermined temperature in the 45 to 60°C range; (b) mixing a defined amount of the liquid fat with a water-soluble solvent for androstenone at the temperature of the liquid; (c) cooling the fat/solvent mixture down to a predetermined temperature, at which a substantial proportion of the fat dissolved in the solvent is separated from the solution whereas the major part of the androstenone dissolved in the solvent phase remains dissolved in the solvent; (d) sampling of a defined amount of the androstenone-containing solvent phase and diluting it in a predetermined ratio with an aqueous buffer solution admissible for the used detection process; (e) de- termining the androstenone content of the solvent/buffer solution phase by means of known per se competitive immunological detection reactions.
Claus Rolf Paul
Dehnhard Martin
Fetherstonhaugh & Co.
Riedel-De Haen Aktiengesellschaft
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