Process for determining superoxide dismutase activity

Details Process for determining superoxide dismutase activity Process for determining superoxide dismutase activity

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/26 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 1272943

ABSTRACT OF THE DISCLOSURE Superoxide dismutase activity is determined by measuring hydrogen peroxide produced by using superoxide anion as a substrate and acting superoxide dismutase thereon in the presence of an electron carrier. When maleimide or a derivative thereof, and if necessary, a carbonyl compound, and if further necessary bromine ions and/or citric acid or a salt thereof are added to the electron carrier, the sensitivity and the linearity of calibration curves are further improved.

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