Process for dienone macrolides

Details Process for dienone macrolides Process for dienone macrolides

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) C07H 17/08 (2006.01) C12N 9/08 (2006.01) C12N 15/74 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2191833

The process of this invention is directed to isolating or otherwise obtaining anolefinic macrolide producing microorganism which microorganism does not contain epoxidase enzyme activity. This invention also relates to a process for preparing said olefinic macrolide by fermenting a mutant microorganism lacking epoxidase activity, designated rosX herein, which mutant is obtained from the wild-type microorganism. This invention also relates to a rosX mutant of Micromonospora rosaria, and to any microorganism having the identifying characteristics thereof, said mutant also designated ATCC 55709. This invention also relates to a process for preparing repromicin, the compound of formula (II), Image (II) by mutating a wild-type microorganism capable of producing rosamicin to produce a mutant microorganism lacking epoxidase activity such that repromicin is produced by said mutant microorganism.

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