Process for disintegrating cell dispersions or cell...

C - Chemistry – Metallurgy – 12 – N

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C12N 13/00 (2006.01) B02C 19/18 (2006.01) C12M 3/08 (2006.01) C12N 1/06 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2109722

The invention relates to a process, which can be carried out continuously, for disintegrating cell material in the form of dispersions or suspensions in water for the purpose of obtaining cell constituents. The invention avoids the use of solid ultrasonication activators and the establishment of a particular geometric form for the acoustic irradiation container by particular parameters with regard to the sonotrode angle, the depth of immersion of the sonotrode, the ratio of extent of immersion of the sonotrode to the acoustic irradiation volume and the ratio of extent of immersion of the sonotrode to the solid matter content of the medium to be sonicated. In this process, the solid matter in the cell dispersion or suspension can be in the range from about 0.5 to 65% by weight.

Cette invention concerne un procédé de désintégration, en continu, de tissu cellulaire se présentant sous la forme de dispersions ou de suspensions aqueuses, aux fins d'extraction d'éléments constitutifs. L'objet de l'invention rend inutile l'utilisation d'activateurs d'ultrasonication et la configuration du volume d'irradiation acoustique selon une géométrie particulière faisant intervenir des paramètres particulier quant à l'angle d'implantation du sonotrode, la profondeur de pénétration de celui-ci dans le volume d'irradiation, le rapport de la profondeur de pénétration à la taille du volume d'irradiation et le rapport de la profondeur de pénétration du sonotrode à la teneur en matières solides du milieu à soumettre au traitement de sonication. Le procédé objet de l'invention s'accomode d'une teneur en matières solides de la dispersion ou suspension aqueuse comprise entre environ 0,5 et 65 % en poids.

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