Process for dissolving off an analyte from it's binding protein

Details Process for dissolving off an analyte from it's binding protein Process for dissolving off an analyte from it's binding protein

G - Physics

01

N

150/3, 167/37, 1

G01N 33/48 (2006.01) C07D 339/04 (2006.01) G01N 33/531 (2006.01) G01N 33/74 (2006.01) G01N 33/78 (2006.01) G01N 33/82 (2006.01)

Patent

CA 2007337

ABSTRACT The present invention provides a process for the dissolving off of an analyte bound to a binding protein by breakdown of the binding protein by a thiol cleaving SH groups in the alkaline range, wherein as SH group-cleaving thiol, there is used a 1,2-dithiolan-3-carboxylic acid of the general formula:- Image ( I ) in which n is a whole number of from 1 to 8. The present invention is also concerned with the use of this process for preparing samples for analytical or investigational processes for the determination of a liberated analyte and especially for immunoassays for vitamin B12.

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