Process for enzymatic hydrolysis of pretreated...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/42 (2006.01) C12N 1/20 (2006.01) C12P 19/14 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2661568

An enzyme mixture for the enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars is provided. Also provided is a process for hydrolyzing a pretreated lignocellulosic feedstock with an enzyme mixture. The enzymatic hydrolysis comprises adding a cellulase enzyme mixture to a pretreated lignocellulosic feedstock. The cellulase enzyme mixture comprises a primary cellulase mixture of CBHl and CBH2, and EGl and EG2. The CBHl and CBH2 being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture. Furthermore, CBH2 is present at a fraction relative to CBHl and CBH2, and EG2 is present at a fraction relative to the EGl and EG2.

La présente invention concerne un mélange enzymatique permettant l'hydrolyse enzymatique d'une matière première d'alimentation lignocellulosique prétraitée en sucres solubles. Elle concerne également un procédé permettant d'hydrolyser une matière première d'alimentation lignocellulosique prétraitée au moyen d'un mélange enzymatique. Le procédé d'hydrolyse enzymatique consiste à ajouter un mélange enzymatique de cellulases à une matière première d'alimentation lignocellulosique prétraitée. Le mélange enzymatique de cellulases comprend un mélange de cellulases primaire de CBH1 et de CBH2, et de EG1 et de EG2. La teneur en CBH1 et CBH2 est supérieure ou égale à 55% et inférieure à 85% du mélange de cellulases primaire. CBH2 est présente à hauteur d'une fraction par rapport à CBH1 et CBH2 et EG2 est présente à hauteur d'une fraction par rapport à EG1 et EG2.

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