C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/18 (2006.01) C07H 3/04 (2006.01) C07H 3/06 (2006.01) C12P 19/12 (2006.01) C12P 19/24 (2006.01)
Patent
CA 2220675
The invention concerns an improved method for the enzymatic galactosylation of monosaccharides and oligosaccharides with in situ regeneration of the nucleotide sugar (or the nucleoside diphosphate sugar) in the presence of saccharose synthase, .beta.-1-4-galactosyl transferase and uridine diphosphate glucose-4'-epimerase (UDP glucose-4'-epimerase), the method calling for the uridine diphosphate glucose-4'-epimerase to be reactivated by a ketone sugar derivative.
L'invention concerne un procédé perfectionné de galatosylation enzymatique de monosaccharides et d'oligosaccharides avec régénération in situ du sucre de nucléotide (par ex. du sucre de diphosphate de nucléoside) en présence de saccharose-synthase, de 1-4-galactosyltransférase et d'uridine diphosphate-glucose-4'-épimérase (UDP-glucose-4'-épimérase). Selon ce procédé, l'uridine diphosphate-glucose-4'-épimérase est réactivée à l'aide d'un dérivé de sucre cétonique.
Elling Lothar
Horsch Brigitte
Kula Maria Regina
Marquardt Rudiger
Seiffert-Storiko Andreas
Fetherstonhaugh & Co.
Hoechst Aktiengesellschaft
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