C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/815 (2006.01) C07K 1/113 (2006.01) C07K 1/14 (2006.01) C07K 14/485 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2194177
The invention relates to a process for the preparation of a biologically active and correctly folded protein in the presence of a denaturing agent like urea or guanidine hydrochloride and the separation of the correctly folded protein therefrom directly. This process can be applied, for example, in the renaturation of recombinant proteins like hirudin or epidermal growth factor.
L'invention concerne un procédé permettant de préparer une protéine repliée correctement, biologiquement active, en présence d'un agent dénaturant tel que l'urée ou l'hydrochlorure de guanidine, et de séparer la protéine correctement repliée directement de celui-ci. Ce procédé peut être mis en oeuvre, par exemple, lors de la renaturation de protéines de recombinaison telles que l'hirudine ou le facteur de croissance épidermique.
Ag Ciba-Geigy
Fetherstonhaugh & Co.
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