Process for generating male sterile plants

Details Process for generating male sterile plants Process for generating male sterile plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 1/02 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2164732

The invention is directed to a process for the generation of male sterility in plants comprising the steps of (a) transforming a plant cell with DNA sequences that selectively inhibit the expression of essential metabolic compounds and (b) regenerating plants from said plant cells. Cells impaired in the biosynthesis of basic metabolic compounds undergo starvation and die. Such pathways include amino acid biosynthesis, nucleic acid biosynthesis and other biosynthetic pathways such as citric acid cycle, pentose phosphate pathway, fatty acid metabolism, vitamin biosynthesis that will render the cell inactive due to nutrient depletion, if one or more enzymes or proteins involved in this pathway would become inactive by using inhibitory DNA constructs.

L'invention se rapporte à un procédé permettant d'induire la stérilité dans des plantes mâles, et consistant à (a) transformer une cellule de plante au moyen de séquences d'ADN qui inhibent sélectivement l'expression de composés métaboliques essentiels et (b) régénérer les plantes à partir desdites cellules de plantes. Les cellules endommagés au cours de la biosynthèse de composés métaboliques de base s'étiolent et finissent par mourir. De tels processus comprennent la biosynthèse d'acides aminés, la biosynthèse d'acide nucléique ainsi que d'autres processus de biosynthèse tels que le cycle d'acide citrique, le processus à base de phosphate de pentose, le métabolisme d'acide gras, et la biosynthèse de vitamines, qui vont rendre la cellule inactive par appauvrissement en substances nutritives, si au moins une enzyme ou protéine impliquée dans cette voie est inactivée par l'utilisation d'ADN de recombinaison inhibiteurs.

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