Process for identifying para cation channel modulators

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 16/28 (2006.01) C12Q 1/18 (2006.01) G01N 33/554 (2006.01) G01N 33/60 (2006.01)

Patent

CA 2204849

DNAs encoding voltage-activated cation channels have been cloned and characterized. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the channel activity, and channel modulators are identified. Channel modulators are useful as insecticides and arachnicidic agents.

On a cloné et caractérisé des ADN codant pour des canaux cationiques activés par une tension. Les ADN complémentaires ont été exprimés dans des cellules hôtes de recombinaison, pour produire des protéines de recombinaison actives. Les protéines de recombinaison ont également été purifiées à partir des cellules hôtes de recombinaison. En plus, les cellules hôtes de recombinaison ont été utilisées pour mettre au point un procédé permettant d'identifier des modulateurs de l'activité des canaux et les modulateurs des canaux ont été identifiés. Les modulateurs des canaux sont utiles pour des préparations insecticides et arachnicides.

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