Process for immobilizing nucleic acid probes on polystyrene...

Details Process for immobilizing nucleic acid probes on polystyrene... Process for immobilizing nucleic acid probes on polystyrene...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2125145

2125145 9313224 PCTABS00024 Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water; (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface; and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent; and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.

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