Process for improving efficiency of dna amplification reaction

C - Chemistry – Metallurgy – 07 – H

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C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2433141

The present Invention has an object of providing a process for improving the efficiency of a DNA amplification reaction, and a process for improving the hybridization specificity of an oligonucleotide to a DNA. The present invention provides a process for improving the efficiency of a DNA amplification reaction, wherein a primer in which a compound such as LC-Red 705 or an oligonucleotide with a GC content of at least 25% and with at least four bases is added to the 5' terminus is used as the primer; as well as a process for improving the hybridization specificity of an oligonucleotide to a DNA sample, wherein an oligonucleotide in which a compound such as LC-Red 705 is conjugated to the 5' terminus is used for hybridizing to a DNA.

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