C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/99 (2006.01) C07K 14/815 (2006.01) C12N 9/60 (2006.01) C12N 13/00 (2006.01) C12N 1/19 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2183406
The present invention relates to a process for inacti-vating carboxypeptidase Y in a hirudin-containing culture broth produced by fermenting a transformed yeast, which comprises continuously heating the culture broth, flowing through a tubular apparatus which has a heating-up section, a holding section and a cooling section, to a temperature of 80 to 100°C, preferably 85 to 95°C, particularly preferably to a temperature of 85°C.
Crause Peter
Habermann Paul
Moller Jorg
Ulmer Wolfgang
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Hoechst Aktiengesellschaft
Schering Aktiengesellschaft
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