Process for isolating and purifing ovine hyaluronidase

Details Process for isolating and purifing ovine hyaluronidase Process for isolating and purifing ovine hyaluronidase

C - Chemistry, Metallurgy

12

N

C12N 9/26 (2006.01) C12N 15/56 (2006.01)

Patent

CA 2522544

The disclosure relates to a process for preparing a hyaluronidase preparation suitable for pharmaceutical applications. In a preferred embodiment, the process includes the use of viral filtration steps to increase the purity of the final product. The process provides a method that enhances the purity of hyaluronidase preparations presently available in commerce. The methods are preferably used to purify hyaluronidase from mammalian sources. In an alternative embodiment, the methods disclosed can be used to purify recombinant hyaluronidase.

L'invention concerne un procédé servant à préparer une préparation hyaluronidase destinée à des applications pharmaceutiques. Dans un mode de réalisation préféré, ce procédé consiste à effectuer une filtration virale afin d'augmenter la pureté du produit final. L'invention concerne également un procédé permettant d'augmenter la pureté des préparations hyaluronidase actuellement disponibles dans le commerce. Les procédés selon l'invention sont utilisés de préférence pour purifier l'hyaluronidase d'origine mammifère. Dans un autre mode de réalisation, lesdits procédés peuvent être utilisés pour purifier l'hyaluronidase recombinée.

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