C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/30 (2006.01) C07H 19/10 (2006.01)
Patent
CA 2157457
The present invention relates to an improved process for isolating and purifying sugar nucleotides, in particular cytidine monophosphate-activated N-acetylneuraminic acid (CMP-Nana), from biological sources. The sugar nucleo- tides can be obtained from a sugar nucleotide-containing solution, which has been freed of proteins by alcoholic precipitation, by means of an improved column-chromato- graphic method which uses silica gel as the stationary phase, at a purity which is so great that a subsequent desalting step is all that is required to isolate them.
Horsch Brigitte
Kretzschmar Gerhard
Marquardt Rudiger
Meiwes Johannes
Seiffert-Storiko Andreas
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Kretzschmar Gerhard
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