Process for isolating galactose oxidase

Details Process for isolating galactose oxidase Process for isolating galactose oxidase

C - Chemistry, Metallurgy

12

N

C12N 9/04 (2006.01)

Patent

CA 2069629

A method of isolating and purifying galactose oxidase from a fungus media is disclosed. The supernatant liquid from the fermentation broth is subjected to ultrafiltration and then separated on a carboxy-sulfon cation exchange column using high pres- sure liquid chromatography. Copper ions must be present to insure enzyme activity.

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