Process for isolating lactoferrin and lactoperoxidase from...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/08 (2006.01) A23C 9/146 (2006.01) A23J 1/20 (2006.01) C07K 1/18 (2006.01) C07K 14/79 (2006.01)

Patent

CA 2128111

The invention relates to a process for isolating bioactive proteins such as lactoperoxidase and lactoferrin from milk and/or milk derivatives, characterized by the steps of: a) adsorbing said proteins to a cation exchanger by passing the milk or the milk derivative over the cation exchanger at a high superficial velocity (more than 500 cm per hour) and a high liquid load (100-600 bed volumes per hour); b) eluting these proteins, separately or simultaneously, by elution with one or more salt solutions, so as to form one or more eluates, optionally followed by c) drying of the said eluates. The invention further relates to products obtained by this process.

L'invention porte sur une méthode pour isoler des protéines bioactives, comme la lactoperoxydase et la lactoferrine, à partir du lait et (ou) de dérivés du lait. Elle comprend les étapes suivantes : a) adsorption des protéines sur un échangeur cationique en faisant passer le lait ou le dérivé du lait sur un échangeur de ce type à une vitesse superficielle (plus de 500 cm à l'heure) et à charge liquide élevées (100-600 volumes de lit par heure); b) élution de ces protéines, séparément ou simultanément, par élution avec une ou plusieurs solutions de sel, de façon à former un ou plusieurs éluats; c) facultativement, séchage des éluats. L'invention porte également sur des produits obtenus grâce à ce procédé.

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