Process for isolating microorganisms from samples and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/24 (2006.01) B03C 1/02 (2006.01) C12N 1/02 (2006.01) C12Q 1/25 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2685229

A process for isolating microorganisms from samples, particularly Shigella spp. from food samples, and a system, apparatus and composition therefor are provided. Magnetic particles are used to capture microorganisms and a system having separate magnetically-based apparatuses for collecting, concentrating and retrieving is used to isolate the magnetic particles having bound microorganisms. The apparatus for concentrating magnetic particles utilizes a small magnet assisted by vibration to concentrate collected particles at a localized region on the bottom of a container. The process, system and apparatus of the present invention are simple and inexpensive providing improved magnetic particle recovery adaptable to large scales.

L'invention concerne un procédé destiné à isoler des microorganismes à partir d'échantillons et, en particulier, à isoler Shigella spp. à partir d'échantillons alimentaires et un système, un appareil et une composition à cet effet. L'invention consiste à utiliser des particules magnétiques pour capturer des microorganismes et à utiliser un système présentant des appareils magnétiques séparés destinés à recueillir, concentrer et récupérer les particules magnétiques, afin d'isoler les particules magnétiques présentant des microorganismes liés. L'appareil destiné à concentrer les particules magnétiques utilise un petit aimant assisté par vibration pour concentrer les particules recueillies sur une zone localisée, au fond d'un récipient. Le procédé, le système et l'appareil de la présente invention sont simples et économiques, et permettent une récupération de particules magnétiques adaptable à grande échelle.

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