C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2394701
The present invention relates to a process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of: providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.
L'invention concerne un procédé de fragmentation et de marquage d'un acide nucléique naturel ou synthétique, comprenant les étapes suivantes : fournir un mélange contenant un acide nucléique, un agent de marquage contenant un marqueur détectable, et au moins un cation métallique polyvalent dans une solution sensiblement aqueuse ; fragmenter chimiquement l'acide nucléique dans le mélange pour produire une multiplicité de fragments d'acide nucléique ; et apposer au moins un marquage à au moins un des fragments d'acide nucléique pour produire un fragment d'acide nucléique à marquage détectable.
Banerjee Aloke Raj
Becker Michael M.
Browne Kenneth A.
Friedenberg Matthew C.
Hajjar Fred F.
Bio Merieux
Gen-Probe Incorporated
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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