C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2394392
The present invention relates to a process for labeling with signal amplification a ribonucleic acid (RNA), characterized in that it comprises: fragmenting the RNA, fixing a first ligand to the terminal phosphate which is located at the 3' end and/or the 5' end of each fragment of said RNA, said terminal phosphate having been released during the fragmentation, and binding a labeling agent to said first ligand. The invention is preferably applied in the field of medical diagnosis.
La présente invention concerne un procédé de marquage avec amplification de signal d'un acide ribonucléique (ARN), consistant à fragmenter l'ARN, à fixer un premier ligand au phosphate terminal situé à l'extrémité 3' end et/ou à l'extrémité 5' de chaque fragment dudit ARN, ledit phosphate terminal ayant été libéré lors de la fragmentation, et à fixer un agent de marquage au premier ligand. L'invention est, de préférence, utilisée dans le domaine du diagnostic médical.
Do Duc
Laayoun Ali
Miyada Charles Garret
Affymetrix Incorporated
Bio Merieux
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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