Process for lysing mycobacteria

C - Chemistry – Metallurgy – 12 – N

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C12N 1/06 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2135271

A process for lysing Mycobacteria to liberate nucleic acids such as DNA and RNA comprises heating the Mycobacteria in an aqueous solution to lyse the Mycobacteria and release the nucleic acids, with the aqueous solution containing a chelating agent such as EDTA or EGTA in an amount effective to inhibit degradation of the released nucleic acids. Examples of Mycobacteria which can be lysed include Mycobacterium fortuitum and Mycobacterium tuberculosis. After lysis, the nucleic acid is preferably then amplified and detected. Kits useful for carrying out the present invention are also disclosed.

Procédé de lyse de mycobactéries pour libérer des acides nucléiques, comme l'ADN et l'ARN, consistant à chauffer les mycobactéries dans une solution aqueuse pour la lyse des mycobactéries et la libération d'acides nucléiques; la solution aqueuse renferme un agent chélateur, notamment l'EDTA ou l'EGTA en quantités suffisantes pour empêcher la dégradation des acides nucléiques libérés. Exemples de mycobactéries pouvant se prêter à la lyse : Mycobacterium fortuitum et Mycobacterium tuberculosis. On privilégie l'amplification et la détection de l'acide nucléique après la lyse. On présente également des trousses utiles pour mettre en oeuvre le procédé de la présente invention.

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