Process for lysing mycobacteria

Details Process for lysing mycobacteria Process for lysing mycobacteria

C - Chemistry, Metallurgy

12

N

C12N 1/06 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2084533

The invention provides a rapid process for lysing Mycobacteria. In one embodiment is provided a process for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of heat. The process of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.

La présente invention concerne un procédé rapide pour lyser des Mycobactéries. Dans une réalisation, le procédé consiste à exposer les bactéries à une quantité de chaleur efficace pour provoquer la lyse. Ce procédé est particulièrement avantageux puisqu'il y a seulement une étape. Il est très rapide comparativement aux méthodes antérieures et nécessite peu d'instruments et d'efforts. De plus, ce procédé permet d'isoler des composants cellulaires, y compris l'acide désoxyribo-nucléique (ADN), des Mycobactéries. L'isolement de l'ADN permet une analyse postérieure par hybridation au moyen de sonde et une analyse des enzymes de restriction.

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