Process for making 1,3-propanediol from carbohydrates using...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/18 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/60 (2006.01)

Patent

CA 2220396

The present invention provides a process for the biotransformation of a carbohydrate carbon source to 1,3-propanediol using mixed yeast and bacterial cultures wherein the carbohydrate is first fermented to glycerol by the yeast cell and then converted to 1,3-propanediol by the bacterial cell containing an active diol or glycerol dehydratase enzyme. In this process both the yeast and bacterial cultures are supported on the same carbon source, and 1,3-propanediol is isolated from the media.

La présente invention se rapporte à un procédé de biotransformation d'une source de carbone constituée d'hydrates de carbone en un 1,3-propanediol à l'aide d'un mélange de levure et de cultures bactériennes. Les hydrates de carbone subissent d'abord une fermentation au contact avec les cellules de levure, ce qui produit du glycérol, puis sont transformés en un 1,3-propanediol par les cellules bactériennes contenant un diol actif ou une enzyme du type glycérol déshydratase. Dans ce procédé, la levure et les cultures bactériennes sont portées par la même source de carbone, et le 1,3-propanediol est isolé du milieu de culture.

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