C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12M 1/40 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01) C12S 3/20 (2006.01)
Patent
CA 2156231
A process for making the product of a nucleic acid amplification reaction, which amplification reaction employs one or more primer pairs, incapable of being a target for further amplification comprising: contacting the amplified product with an oligonucleotide capable of hybridizing to a stretch a nucleotides of the amplified product, said stretch being situated between the hybridisation sites of a pair of primers, under conditions which allow formation of a hybridisation complex between the oligonucleotide and the amplified product to occur, said oligonucleotide being modified in such a way that it protects the double stranded part of the hybridisation complex hybridized to the oligonucleotide from degradation, and subjecting the hybridisation complex to a degradative treatment under circumstances such that at least the part of the hybridisation complex capable of hybridizing to the primers is degraded. An assay for diagnosing the presence of a nucleic acid sequence in a sample comprising carrying out the afore-mentioned method and a kit for carrying out the afore-mentioned method.
Schukkink Adriana Frederieke
Van Gemen Bob
Akzo Nobel Nv
Fetherstonhaugh & Co.
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