Process for manufacture of pyruvate oxidase and its use for...

C - Chemistry – Metallurgy – 12 – N

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195/123, 150/15.

C12N 9/02 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 1143640

ABSTRACT OF THE DISCLOSURE Pyruvate oxidase is produced by a process which comprises culturing A pyruvate oxidase-producing microorganism belonging to the genus Pediococcus, Streptococcus or Aerococcus in a nutrient culture medium, and isolating the pyruvate oxidase thus produced from the culture. also provided by the invention disclosed herein is an analytical method for pyruvic acid in a sample containing pyruvic acid or a system which liberates this acid, which comprises treating said sample with a reaction system containing pyruvate oxidase, and measuring the consumed component or generated component.

324045

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