Process for maximizing the growth and nicotine degrading...

Details Process for maximizing the growth and nicotine degrading... Process for maximizing the growth and nicotine degrading...

C - Chemistry, Metallurgy

12

P

195/49, 195/59

C12P 1/00 (2006.01) A24B 15/20 (2006.01) C12N 1/04 (2006.01) C12P 17/12 (2006.01)

Patent

CA 1067027

PATENT APPLICATION OF LAWRENCE E. GRAVELY VERNON L. GEISS and RICHARD P. NEWTON FOR PROCESS FOR MAXIMIZING THE GROWTH AND NICOTINE DEGRADING ACTIVITY OF MICROORGANISMS Abstract of Disclosure A process is disclosed for maximizing the growth and nicotine degrading activity of microorganisms effective to degrade nicotine through a biochemical mechanism in which 3-succinoylpyridine is formed. The process involves inocu- lating a nicotine-containing growth medium with the micro- organisms and subjecting the broth to aeration and agitation. During the growth period, the broth should be maintained at a pH of between about 6 and 7.8 and at a temperature between about 10°C and about 45°C. The broth should have an initial nicotine concentration of at least 0.5 mg. per ml. up to an amount which is toxic to the microorganisms.

265035

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