Process for obtaining growth factor preparations (tgf-beta...

Details Process for obtaining growth factor preparations (tgf-beta... Process for obtaining growth factor preparations (tgf-beta...

C - Chemistry, Metallurgy

07

K

C07K 14/495 (2006.01) A23J 1/20 (2006.01) C07K 14/65 (2006.01)

Patent

CA 2387686

The present invention relates to a process for extracting transforming growth factor .beta. (TGF-.beta.) and insulin-like growth factor 1 (IGF-1) from a milk product, comprising the steps of: a) recovering a basic fraction from the milk product by means of cationic exchange chromatography; b) passing the fraction obtained in step a) over a hydroxyapatite column; c) eluting the hydroxyapatite column with appropriate eluents in such a way as to obtain two separate fractions, these fractions being: i) a fraction comprising IGF-1 in the substantial absence of TGF-.beta.; ii) a fraction comprising TGF-.beta. in the substantial absence of IGF-1. This process can comprise a further step d) in which a lactoperoxidase fraction can be obtained. The invention further relates to the products obtained with this process.

La présente invention concerne un procédé d'extraction de facteur de croissance transformant .beta. (TGF-.beta.) et de facteur de croissance analogue à l'insuline (IGF-1) à partir d'un produit laitier. Le procédé comporte les étapes consistant à : a) récupérer une fraction de base du produit laitier par chromatographie à échange cationique ; b) faire passer la fraction obtenue à l'étape a) sur une colonne d'hydroxyapatite ; c) éluer la colonne d'hydroxyapatite à l'aide d'éluents appropriés de manière à obtenir deux fractions séparées, ces fractions étant : 1) une fraction contenant IGF-1 et sensiblement exempte de TGF-.beta. ; 2) une fraction contenant TGF-.beta. et sensiblement exempte d'IGF-1. Le procédé peut comporter une étape supplémentaire d), dans laquelle une fraction de lactoperoxidase peut être obtenue. L'invention concerne en outre les produits obtenus à l'aide du procédé.

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