Process for obtaining ligninolytic enzymes

Details Process for obtaining ligninolytic enzymes Process for obtaining ligninolytic enzymes

C - Chemistry, Metallurgy

12

N

195/123

C12N 9/00 (2006.01) C12N 1/38 (2006.01) C12N 9/08 (2006.01) C12N 9/14 (2006.01)

Patent

CA 2006490

ABSTRACT The extraction of ligninolytic enzymes by cultivation of white- rot fungi can be improved in that at the point of incipient colouration at least 0.1 g/l, in particular 0.3 to 5 g/l, and especially 1 g/l of manganese dioxide powder, in particular finely divided powder with a grain size of less than 10 µm, is added to the fungal culture and the pH value in the medium is kept constant at a value between 4.8 and 5.0 during the whole of the culturing period. It is preferred that the enzymes be periodically separated off from the culture medium by ultra- filtration and that the residual culture substrate be recycled.

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