Process for plasmid dna fermentation

Details Process for plasmid dna fermentation Process for plasmid dna fermentation

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01)

Patent

CA 2576939

Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature Fed- batch fermentation strategies, combined with novel growth and induction phase temperature shifts.

Des améliorations apportées à la technologie de production de l'ADN plasmidique sont nécessaires pour assurer la faisabilité économique de futurs vaccins à ADN et d'agents thérapeutiques d'ADN. L'invention concerne des procédés généraux, au moyen desquels il est possible d'augmenter considérablement la productivité d'ADN plasmidique dans un fermenteur. Ces procédés impliquent des stratégies de fermentation à écoulement discontinu, combinées à des nouveaux changements de température de temps de latence et de croissance.

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