C - Chemistry – Metallurgy – 08 – F
Patent
C - Chemistry, Metallurgy
08
F
204/99.02
C08F 2/54 (2006.01) B01D 57/02 (2006.01) C08F 20/56 (2006.01) G01N 27/447 (2006.01)
Patent
CA 1285902
IP-0417-1 TITLE PROCESS FOR PREPARATION OF ELECTROPHORESIS GEL MATERIAL Abstract Of The Disclosure Describes a process for preparing a porous gel product defining a volume useful for electrophoretic separation the gel product being characterized by the presence of a photoinitiator, having a constant atomic composition over its volume, and length and width dimensions, and which is stable, reproducible and has a controlled porosity gradient along one of the length and width dimensions by the steps of: forming a water solution or dispersion of a mixture of from about 3% weight/volume to about 30% weight/volume of acrylamide monomer and a cross-linking agent, the cross-linking agent comprising about 2% weight/weight to about 15% weight/weight of the total monomer, wherein the cross-linking agent consists essentially of N,N'-(1,2,-dihydroxyethylene)-bis-acrylamide, N,N'-methylene-bis-acrylamide (BIS), N,N'- diallyltartramide (DATD), ethylenediacrylate, N,N'-bis, acrylylcystamine, and polyoxyethyltri- methylolpropane triacrylate (TEOTA), adding a free radical generating system that absorbs ultraviolet light radiation and initiates polymerizatin of the monomers, the free radical generating system consisting essentially of aqueous soluble or dispersible compounds, forming the solution into the shape of the desired gel product, subjecting the formed solution to ultraviolet radiation to polymerize and cross-link the monomer solution or dispersion, and modulating the exposure of the formed solution to the ultraviolet radiation over the length and/or width of desired gel product according to the desired porosity gradient.
588476
E. I. Du Pont de Nemours And Company
Sim & Mcburney
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