Process for preparation of human glicentin

Details Process for preparation of human glicentin Process for preparation of human glicentin

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/16 (2006.01) C07K 14/605 (2006.01) C12N 15/16 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2139155

Disclosed is a process for the preparation of native human glicentin, which comprises producing by gene technology a fused protein composed of human glicentin having a peptide moiety connected to the N-terminus of human glicentin and removing the connected peptide moiety from the fused protein, the improvement in which a purification of the fused protein and human glicentin is carried out by utilizing a reversible covalent bond of a cystein residue with the stationary phase of a chromatography.

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