Process for preparing 2-keto-l-gulonic acid

C - Chemistry – Metallurgy – 12 – P

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C12P 7/60 (2006.01) C12P 7/58 (2006.01) C12P 39/00 (2006.01)

Patent

CA 1186650

Abstract of the Disclosure 2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on a medium containing D-glucose, employing two kind of microorganisms; the first, a 2,5-diketo-D-gluconic acid producing microorganism which belongs to genus Erwinia and the second, a 2-keto-L-gulonic acid producing microorganism which belongs to genus Brevibacterium or Corynebacterium. The incubation of the microorganisms in a medium containing D-glucose is used in the disclosed process. By-production of 2-keto-D-gluconic acid, the undesired isomer of the intended product is effectively prevented by employing the mixed culturing because of the co-existence of the both microorganisms in the medium during at least part of the entire cultivation. Namely, 2-keto-D-gluconic acid produced by the second microorganism is utilized by the first microorganism to produce 2,5-diketo-D-gluconic acid which is subsequently converted into 2-keto-L-gulonic acid by the second microorganism. - 23 -

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