Process for preparing a protein by a fungus transformed by...

Details Process for preparing a protein by a fungus transformed by... Process for preparing a protein by a fungus transformed by...

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C12N 9/20 (2006.01) C12N 9/40 (2006.01) C12N 15/13 (2006.01) C12N 15/52 (2006.01) C12N 15/80 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2063592

A process is disclosed for preparing a protein by a eukaryote transformed by multicopy integration of an expression vector into the genome of a yeast, such as Saccharomyces, Hansenula and Kluyveromyces, or of a mould such as Aspergillus, Rhizopus and Trichoderma, said expression vector containing both an "expressible gene" encoding said protein and a so-called "deficient selection marker needed for the growth of the yeast or mould in a specific medium", such as the LEU2d, TRP1d or URA3d gene, in combination with a ribosomal DNA sequence, resulting in stable high copy integration of 100-300 copies per cell. This multicopy integration results in a increased production of the desired protein, which can be guar a-galactosidase, an oxidase or a hydrolytic enzyme such as a lipase.

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