Process for preparing an improved western blot immunoassay

Details Process for preparing an improved western blot immunoassay Process for preparing an improved western blot immunoassay

G - Physics

01

N

167/44

G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2016313

The present invention encompasses an improved immunoassay method which involves the simultaneous transfer of multiple antigens onto a single solid support. The antigens which may be utilized by this method may be either naturally or recombinantly produced arid may be purified on a variety of electrophoresis gels. This method is particularly useful to provide an extremely sensitive multiple component assay using essentially pure antigenic polypeptides capable of detecting the presence of antibodies to HIV-1 or HIV-2 viruses in infected patients.

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