Process for preparing and purifying an anticoagulant

Details Process for preparing and purifying an anticoagulant Process for preparing and purifying an anticoagulant

C - Chemistry, Metallurgy

07

K

530/13

C07K 14/435 (2006.01) C07K 1/30 (2006.01) C07K 1/36 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2021147

Abstract Process for Purifying Annexines The process comprises the steps of a) adjusting the pH value of homogenised cellular material to 8.0 to 10.0; b) adding at least one bivalent cation selected from Ca2+, Cd2+, Zn2+, Mn2+ and Co2+; c) adding a phospholipid; d) washing insoluble cell residue to remove soluble constituents; and e) extracting annexines from the cell residue using chelating agents. Overall yields of annexines are raised to more than 80%.

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