Process for preparing biological mammalian implants

Details Process for preparing biological mammalian implants Process for preparing biological mammalian implants

A - Human Necessities

61

F

167/104, 195/59

A61F 2/24 (2006.01) A01N 1/02 (2006.01) A61L 27/36 (2006.01)

Patent

CA 1261290

ABSTRACT OF THE DISCLOSURE A process for preparing biological material for implant in mammal's cardiovascular system, respiratory system or soft tissue is disclosed. The process comprises: 1) isolating from a suitable donor a desired tissue sample of the biological material; 2) extracting the tissue sample with an hypotonic buffer solution at a mild alkaline pH, the buffer solution including active amounts of proteolytic inhibitors and antibiotics; 3) extracting the tissue sample with a buffered solution having a high concentration of salt, the solution being at a mild alkaline pH and including a non-ionic detergent with protease inhibitors and antibiotics; 4) subjecting tissue sample to enzymatic digestion in a buffered saline solution, the enzymes consisting of purified protease-free deoxyribonuclease and ribonuclease; 5) extracting the tissue sample with an anionic detergent at a mild alkaline pH; and 6) storing the tissue sample in physiologic buffered solutions.

543031

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