Process for preparing culture mediums usable for culturing...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/18 (2006.01) A21D 8/04 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2149458

A culture medium which is completely free of chemical ad- ditives and which can be used for the individual culture of yeast and of lactic acid bacteria or for the coculture of yeasts and lactic acid bacteria is prepared by a process comprising the steps of: preparing a diluted aqueous mixture comprising at least whole-meal and/or wheat germs as well as yeast autolysate; completely hydrolyzing the starch in this mixture into fermentable sugars by the action of at least one alpha-amylase and of at least one amyloglucosidase; gently hy- drolyzing in this mixture at least part of the gluten by means of proteolytic enzymes of food quality into aromatic peptides and into free amino acids, and this in sufficient quantity to assure, when using the culture medium, a maximum microbial growth; and sterilizing the obtained product. The obtained product can be used directly for bread making.

L'invention porte sur un milieu de culture complètement dépourvu d'additifs chimiques qui peut servir à la culture individuelle de levures ou de bactéries lactiques ou à la co-culture de levures et de bactéries lactiques et qui est préparé par un procédé comportant les étapes suivantes : préparation d'un mélange aqueux dilué comprenant au moins de la farine de blé entier et (ou) des germes de blé ainsi que de la levure autolysée; hydrolyse complète de l'amidon de ce mélange en sucres fermentescibles, par l'action d'au moins une alpha-amylase et d'au moins une amyloglucosidase; hydrolyse modérée d'au moins une partie du gluten de ce mélange, au moyen d'enzymes protéolytiques de qualité alimentaire, en peptides aromatiques et en acides aminés libres et ce, en quantité suffisante pour obtenir avec le milieu de culture une croissance microbienne maximale; et stérilisation du produit résultant de ces opérations. Le produit ainsi obtenu peut être utilisé tel quel pour la panification.

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