Process for preparing human coagulation factor viii protein...

Details Process for preparing human coagulation factor viii protein... Process for preparing human coagulation factor viii protein...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 15/57 (2006.01)

Patent

CA 2078721

- 57 - Abstract of the Disclosure: Process for Preparing Human Coagulation Factor VIII Protein Complex A plasmid for expression of human coagulation factor VIII H-chain, a plasmid for expression of human coagulation factor VIII L-chain, an animal cell transformed with either said H-chain expression plasmid or said L-chain expression plasmid or with both thereof, and a process for preparing a human coagulation factor VIII protein complex which comprises forming a transformed animal cell by introducing both said H-chain expression plasmid and said L-chain expression plasmid into said animal cell, culturing said cell to produce the human coagulation factor VIII protein complex in the culture medium and collecting the same. The process of the present invention allows for the production of a safe factor VIII at a high expression level applicable for the production of Factor VIII on an industrial scale.

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