Process for preparing veterinary acellular vaccines against...

Details Process for preparing veterinary acellular vaccines against... Process for preparing veterinary acellular vaccines against...

A - Human Necessities

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A61K 39/02 (2006.01) A61K 39/102 (2006.01) C07K 14/195 (2006.01)

Patent

CA 1327749

-14- PROCESS FOR PREPARING VETERINARY ACELLULAR VACCINES AGAINST GRAM-NEGATIVE NONENTERIC PATHOGENIC BACILLI Abstract of the Disclosure Surface polysaccharide and somatic polysaccharide are recovered from gram-negative nonenteric pathogenic bacilli in purified forms that are useful as veterinary acellular vaccines either individually or combined. The surface polysaccharide recovery process involves shearing the surface polysaccharide and somatic polysaccharide from the cell membrane, removing the bacteria, isolating the surface polysaccharide by precipitation with Cetavlon, solubilizing the precipitate, and removing nucleic acids by ethanol precipitation. The somatic polysaccharide is recovered from the supernatant remaining after the Cetavlon precipitation by ethanol precipitation.

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