Process for preparing viruses and viral vectors

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/86 (2006.01) A61K 31/70 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C07K 14/015 (2006.01) C07K 14/075 (2006.01) C12N 7/00 (2006.01) C12N 9/50 (2006.01)

Patent

CA 2194465

The invention concerns a process for preparing viruses and viral vectors by the transfection of mammal cells with complexes of viral DNA and a polycation, which is optionally conjugated with a ligand for the target cell, in the presence of an endosomolytic agent. The process affords particular advantages for the production of recombinant retroviruses, adenoviruses and adeno- associated viruses in the cell, permitting high virus titres in packing cell lines and in primary cells. The area of application is above all gene therapy.

Procédé de préparation de virus et de vecteurs viraux par transfection de cellules de mammifères avec des complexes d'ADN viral et d'un polycation qui, éventuellement, est conjugué avec un ligand pour la cellule cible, en présence d'un agent endosomolytique. Le procédé offre des avantages particuliers pour la production de rétrovirus de recombinaison, d'adénovirus et de virus adéno-associés dans la cellule, ce qui permet d'obtenir des titres élevés en virus dans des lignées cellulaires d'encapsidation ainsi que dans des cellules primaires. Le domaine d'application concerne principalement la thérapie génique.

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