Process for producing an insulin

Details Process for producing an insulin Process for producing an insulin

C - Chemistry, Metallurgy

07

K

530/7.4

C07K 1/06 (2006.01) C07K 1/12 (2006.01) C07K 14/62 (2006.01)

Patent

CA 1155109

X-5331 Abstract of the Disclosure A process for the production of insulin or an insulin analog is provided by combination of an A-chain and a B-chain, which comprises reacting the S-sulfonated form of the A-chain, the S-sulfonated form of the B- chain, and a thiol reducing agent together in an aqueous medium under conditions which produce a mixture having (1) a pH of from about 8 to about 12, (2) a total protein concentration of from about 0.1 to about 50 milligrams per milliliter, and (3) an amount of thiol reducing agent which affords a total of from about 0.4 to about 2.5 -SH groups per each -SS03 ? group present in the total amount of A- and B-chain S-sulfonates, and allowing formation of insulin or an insulin analog to occur by maintaining the mixture at a temperature of from about 0°C. to about 25°C. and in an environment which provides a source of oxygen.

373706

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