C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 17/04 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01)
Patent
CA 2044911
Disclosed is a process for producing ascorbic acid-2-phosphate which comprises the steps of reaction, in aqueous medium, of ascorbic acid with a phosphate donor in the presence of cells, treated cells, a culture broth or a treated culture broth, of a microorganism belonging to the genus Escherichia and carrying a recombinant DNA comprising a gene isolated from a microorganism belonging to the species Pseudomonas azotocolligans and coding for ascorbic acid-phosphorylating enzyme until a recoverable amount of ascorbic acid-2-phosphate is accumulated in the aqueous medium, and recovery of ascorbic acid-2-phosphate therefrom.
Préparation de l'acide 2-phosphate-ascorbique, consistant à faire réagir en milieu aqueux de l'acide ascorbique avec un donneur phosphate en présence de cellules, de cellules traitées, d'un bouillon de culture ou d'un bouillon de culture traité d'un microorganisme appartenant au genre Escherichia, porteur d'ADN recombinant renfermant un gène isolé d'un microorganisme appartenant à l'espèce Pseudomonas azotocolligans et codant l'enzyme de phophorylation de l'acide ascorbique, jusqu'à accumulation d'une quantité récupérable d'acide 2-phosphate-ascorbique dans le milieu aqueux, et récupération de l'acide 2-phosphate-ascorbique de ce milieu.
Fujio Tatsuro
Koizumi Satoshi
Maruyama Akihiko
Teshiba Sadao
Goudreau Gage Dubuc
Kyowa Hakko Kogyo Co. Ltd.
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