Process for producing cdna

Details Process for producing cdna Process for producing cdna

C - Chemistry, Metallurgy

12

N

195/1.12

C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2003500

The process includes the steps of: a) contacting a liquid containing mRNA with an insoluble support having DNA probes attached thereto via the 5'-terminus thereof whereby the mRNA is hybridised to said probes and hence to said support; b) removing said liquid; and c) adding enzymes and nucleotides in solution whereby the probe functions as a primer to produce single stranded cDNA on the mRNA templates. The DNA probe is oligo-dT or a specific DNA seguence which may correspond to a conserved region in a class of mRNA molecules. The insoluble support may consist of magnet.ic particles which are monodisperse and/or superparamagnetic.

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