C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/1.29, 195/1.
C12P 21/00 (2006.01) C07K 14/57 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01)
Patent
CA 1321766
Abstract of the Disclosure A process for producing a cell having a high productivity of a desired protein, which comprises (1) transfecting a higher eucaryotic cell which contains a chromosomal DNA coding for dihydrofolate reductase sensitive to methotrexate and functions normally with a gene I coding for a desired protein, a gene II coding for dihydrofolate reductase from a wild type cell and a gene III coding for a protein necessary for the selection of a transformant, and (2) culturing the transformant in the presence of a competitive inhibitor to the dihydrofolate reductase activity to amplify the gene I together wigh the amplification of the gene II, and a process for preparing a desired protein by culturing the thus obtained cell having a high productivity of a desired protein.
522981
Asahi Takashi
Kakutani Tetsu
Kawaharada Hajime
Nakagawa Kaku
Niwa Hideo
Kanegafuchi Kagaku Kogyo Kabushiki Kaisha
Osler Hoskin & Harcourt Llp
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