Process for producing cis-3-hydroxy-l-proline

Details Process for producing cis-3-hydroxy-l-proline Process for producing cis-3-hydroxy-l-proline

C - Chemistry, Metallurgy

12

N

C12N 9/02 (2006.01) C12P 13/24 (2006.01)

Patent

CA 2131776

Provided is a process for industrially producing cis-3-hydroxy-L-proline useful as a raw material for medicines or as an additive to foods. L-proline is converted into cis- 3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is recovered from the aqueous medium. Also provided is a novel enzyme L-proline-3- hydroxylase, which is useful for the process.

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