Process for producing cmp-n-acetylneuraminic acid

Details Process for producing cmp-n-acetylneuraminic acid Process for producing cmp-n-acetylneuraminic acid

C - Chemistry, Metallurgy

12

P

C12P 19/32 (2006.01)

Patent

CA 2540043

A method by which high-purity CMP-N-acetylneuraminic acid (HPLC purity, 95% or higher), which has been difficult to obtain with any technique other than chromatography, can be easily obtained in satisfactory yield by a simple operation without the need of chromatography. The process, which is for producing high-purity CMP-N-acetylneuraminic acid (CMP-NeuAc), is characterized by conducting a suitable combination of the following steps (1) to (4). Step 1: a step in which divalent cations are added to a solution containing CMP-NeuAc to thereby precipitate the phosphoric acid, pyrophosphoric acid, and nucleotide which coexist; Step 2: a step in which a phosphatase is added to a solution containing CMP-NeuAc to thereby convert the coexistent nucleotide into nucleoside; Step 3: a step in which an organic solvent is added to precipitate the CMP-NeuAc; and Step 4: a step in which the CMP-NeuAc precipitated is recovered.

L'invention concerne un procédé permettant d'obtenir facilement un CMP-acide N-acétyl-neuraminique de grande pureté (supérieure ou égale à 95%), ceci étant difficile à obtenir avec une autre technique différente de la chromatographie, à un rendement satisfaisant lors d'une opération simple et sans utiliser la chromatographie. Le procédé permettant de produire un CMP-acide N-acétyl-neuraminique (CMP-NeuAc) est caractérisé par le fait qu'il comprend les étapes suivantes (1) à (4). : (1) les cations divalents sont ajoutés à une solution contenant le CMP-NeuAc, ce qui permet de précipiter l'acide phosphorique, l'acide pyrophosphorique et le nucléotide qui coexistent ; (2) une phosphatase est ajoutée à une solution contenant le CMP-NeuAc, ce qui permet de convertir le nucléotide coexistant en un nucléoside ; (3) un solvant organique est ajouté au précipité du CMP-NeuAc ; et (4) le CMP-NeuAc précipité est récupéré.

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